Plasmid

Part:BBa_K5048019

Designed by: Yecheng Zhao   Group: iGEM24_WHU-China   (2024-09-13)


plasmid lptE

This plasmid uses pET-28a as backbone. full-length lptE(signal peptide included) is fused with a FLAG tag. The fusion is activated by T7 promoter. Once expressed, the full-length lptE will anchor in the inner leaflet of the outer membrane, which is unable to be displayed on the surface. We use this plasmid as negative control in our surface display system

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5792
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5792
    Illegal NotI site found at 5817
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5792
    Illegal BglII site found at 4964
    Illegal BamHI site found at 5786
    Illegal XhoI site found at 5826
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5792
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5792
    Illegal NgoMIV site found at 137
    Illegal NgoMIV site found at 3184
    Illegal NgoMIV site found at 3344
    Illegal NgoMIV site found at 4932
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2263


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